If you hosted it in localhost systems,then it is so simple,after clicking on registration button,u need to store the data into database by using
SqlCommand cmd = new SqlCommand("insert into Registration(UserID,FirstName,LastName,Address,ContactNo,DOB,Gender,EmailID) values('" + Label17.Text + "','" + TextBox2.Text + "','" + TextBox3.Text + "','" + TextBox4.Text + "','" + TextBox5.Text + "','" + TextBox6.Text + "','" + RadioButtonList1.SelectedItem.Text + "','" + TextBox7.Text + "')", con);