Try:
Using con As New MySqlConnection(strConnect)
con.Open()
Using com As New MySqlCommand("INSERT INTO myTable (myColumn1, myColumn2) VALUES (@C1, @C2)", con)
com.Parameters.AddWithValue("@C1", myValueForColumn1)
com.Parameters.AddWithValue("@C2", myValueForColumn2)
com.ExecuteNonQuery()
End Using
End Using