By using updatepanel the selected content will get refresh,but fileuploader is not working
That is a known thing. Fileupload control does not work with Update Panel.
There are two options using UpdatePanel,
1) either have a complete postback just in the case of file upload using PostbackTrigger
OR
2) use AsyncFileUpload control, look here:
AsyncFileUpload Demonstration[
^]
OR
Using jQuery, look at this sample and try:
Tutorials:Multiple File Upload Magic[
^]
Ajax File Upload[
^]
AJAX Multiple File Upload Form Using jQuery[
^]