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please give me any idea
You might be using an event to add item(a button perhaps). If yes, use onclick event of the button. In that event create a new listitem(a listbox is a collection of listitems) and use ListBox's Add event to add the new item at the specified position. Give it a try. For a start:
ListBox.Item.Insert(int index, ListItem item);
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its very urgent
I don't think so :-)