My Error was:
Server Error in '/WebSite5' Application.
Access to the path 'E:\Program Files\ImageUp\WebSite5\Upload' is denied.
Description: An unhandled exception occurred during the execution of the current web request. Please review the stack trace for more information about the error and where it originated in the code.
Exception Details: System.UnauthorizedAccessException: Access to the path 'E:\Program Files\ImageUp\WebSite5\Upload' is denied.
ASP.NET is not authorized to access the requested resource. Consider granting access rights to the resource to the ASP.NET request identity. ASP.NET has a base process identity (typically {MACHINE}\ASPNET on IIS 5 or Network Service on IIS 6 and IIS 7, and the configured application pool identity on IIS 7.5) that is used if the application is not impersonating. If the application is impersonating via <identity impersonate="true">, the identity will be the anonymous user (typically IUSR_MACHINENAME) or the authenticated request user.
To grant ASP.NET access to a file, right-click the file in Explorer, choose "Properties" and select the Security tab. Click "Add" to add the appropriate user or group. Highlight the ASP.NET account, and check the boxes for the desired access.
Source Error:
Line 168: Response.ContentType = "application//octet-stream";
Line 169: Response.AppendHeader("Content-Disposition", "attachment; filename=" + fi.Name);
Line 170: Response.TransmitFile(Server.MapPath(fi.Name));
Line 171: Response.End();
Line 172: }
Source File: e:\Program Files\ImageUp\WebSite5\Default.aspx.cs Line: 170
Stack Trace:
[UnauthorizedAccessException: Access to the path 'E:\Program Files\ImageUp\WebSite5\Upload' is denied.]
System.IO.__Error.WinIOError(Int32 errorCode, String maybeFullPath) +9722910
System.IO.FileStream.Init(String path, FileMode mode, FileAccess access, Int32 rights, Boolean useRights, FileShare share, Int32 bufferSize, FileOptions options, SECURITY_ATTRIBUTES secAttrs, String msgPath, Boolean bFromProxy, Boolean useLongPath) +1142
System.IO.FileStream..ctor(String path, FileMode mode, FileAccess access, FileShare share) +83
System.Web.HttpResponse.TransmitFile(String filename, Int64 offset, Int64 length) +132
System.Web.HttpResponse.TransmitFile(String filename) +15
_Default.lnkDownload_Click(Object sender, EventArgs e) in e:\Program Files\ImageUp\WebSite5\Default.aspx.cs:170
System.Web.UI.WebControls.LinkButton.OnClick(EventArgs e) +118
System.Web.UI.WebControls.LinkButton.RaisePostBackEvent(String eventArgument) +113
System.Web.UI.WebControls.LinkButton.System.Web.UI.IPostBackEventHandler.RaisePostBackEvent(String eventArgument) +9
System.Web.UI.Page.RaisePostBackEvent(IPostBackEventHandler sourceControl, String eventArgument) +13
System.Web.UI.Page.RaisePostBackEvent(NameValueCollection postData) +176
System.Web.UI.Page.ProcessRequestMain(Boolean includeStagesBeforeAsyncPoint, Boolean includeStagesAfterAsyncPoint) +5563
Version Information: Microsoft .NET Framework Version:4.0.30319; ASP.NET Version:4.0.30319.272