Hello,
Here's a sample code
<asp:label id="lblOpenid" cssclass="lblOpen" runat="server" text="OpenJobsBySpecificId" xmlns:asp="#unknown">
<asp:textbox id="TextBox1" runat="server" width="78px" xmlns:asp="#unknown">
<asp:button id="Button1" runat="server" text="Click" onclick="Button1_Click" xmlns:asp="#unknown" />
<asp:RequiredFieldValidator ID="reqfTextBox1" Display="None" runat="server" ErrorMessage="Enter Value" ForeColor="red" ControlToValidate="TextBox1" SetFocusOnError="True" CssClass="clsErrorSign" Text="x" Font-Bold="true"></asp:RequiredFieldValidator>
<ajaxToolkit:ValidatorCalloutExtender ID="vcTextBox1" HighlightCssClass="validatorCalloutHighlight" runat="server" TargetControlID="reqfTextBox1">
</ajaxToolkit:ValidatorCalloutExtender>