You can do it with this code:
private void Read_File(object Sender, EventArgs e)
{
string contentOfFileWithCommas = File.ReadAllText("C:\\commas.txt",Encoding.UTF8);
string contentOfFileWithoutCommas = contentOfFileWithCommas.Replace(",", " ");
File.WriteAllText("C:\\nocommas.txt",contentOfFileWithoutCommas,Encoding.UTF8);
}
Hope this helps.