Hi,
I think you are working in windows application so refer the following code....
Here dgvGrid1 --> DataGridView Control.
DataGridViewCheckBoxColumn dgvChkbx = new DataGridViewCheckBoxColumn();
dgvChkbx.DefaultCellStyle.BackColor = Color.Red;
dgvChkbx.DefaultCellStyle.SelectionBackColor = Color.Green;
dgvGrid1.Columns.Add(dgvChkbx);
Cheers :)